Fat-Soluble Vitamins and Related Compounds

S.4.2 Fat-soluble Vitamins and Related Compounds Vitamins Dj and D3 were extracted from milk and were determined using a C column k — 265 nm) and a 97/3 acetonitrile/methanol mobile phase [1120]. They were fully resolved and eluted in <25 min. The authors noted that a 10-min reequilibration time with 97/3 acetonitrile/methanol was needed to generate reproducible results, Whether this was due to late-eluting peaks or some other system... 

Katsui, G. (1972). Pharmaceutical preparations. Fat-soluble vitamins and related compounds. Yukagaku 21 719-724. 

---