Fast GC Quantification of 16 EC Priority PAH Components

Keywords PAH benzo[a] pyrene EU regulation Fast GC high resolution HRMS food meat flavour smoke flavouring 

The best known carcinogenic compound — benzo [ ] pyrene — is used as leading substance out of about 250 different compounds which belong to the PAH 

During GC-MS method setup, it turned out that quadrupole desktop MS instruments could not provide the required selectivity and sensitivity at the low decision level for the list of 16 PAH for a reliable detection in real-life samples. High-resolution mass spectrometry with its particularly high selectivity provided the requested robustness and safety in the quantitative determination (Ziegenhals and Jira, 2006 and 2007 Kleinhenz et al., 2006). 

PAH native Abbreviations Exact mass native (Da) PAH ISTD labelled Exact mass labelled (Da) 

The quantitation was done using isotope dilution technique by the addition of isotope-labelled and fluorinated standards before extraction, as well as for the determination of the response factors of all PAH (Ziegenhals, 2008). Recovery values have been determined by the addition of three deuterium-labelled compounds (Table 4.35). 

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Fast GC Quantification of 16 EC Priority PAH Components 661 Table 4.35 Exact masses of PAH and labelled internal standards. 

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