Screening FACS-based

To address the low throughput limitation associated with most screening methods, various fluorescence-activated cell sorting (FACS) based screening methods have been developed. Unlike the above mentioned screening methods, FACS can analyze and sort up to 100,000 cells per second in a quantitative manner... 

Table 6.2 Summary of the hit data obtained from the CnT-V screening using the FAC-LC/MS method [19], Hits are categorized based on an arbitrary binning strategy, where breakthrough timesa are converted into approximate (fj values. Adapted with permission from the American Chemical Society.

As indicated above, one major advantage of bacterial and other cell-based surface display formats lies in the ability to use fluorescence-activated cell sorting for high-throughput library screening. With modem FACS equipment, such as the Cytomation MoFlo or the FACS Vantage from Beckton-Dickinson, sorting rates of up to 100 000 events per second are possible [10]. 

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