FAB fragments , for

Another approach to increase the loadabihty is to enrich the analyte at the capillary inlet by means of an adsorptive phase, as reviewed in detail in Ref. 10. Re-versed-phase materials such as octadecyl-bonded silica have regularly been used in the form of membranes or column materials, thus, in principle, performing miniaturized SPE ( SPE) in-line with CE, allowing injections of 10-15 /uL. More selective sample concentration is obtained when using antibodies or Fab fragments for coating the inner wall of the capillary inlet [3,5]. 

Lobo E D, Balthasar J P (2005). Application of anti-methotrexate Fab fragments for the optimization of intraperitoneal methotrexate therapy in a murine model of peritoneal cancer. J. Pharm. Sci. 94 1957-1964. 

Brylawski, B. P. Cordeiro-Stone, M. KauBnan, D. G. Ferritin-labeled rabbit Fab fragments for the single-step detection of benzo-a-pyrene-diol-epoxide adducts in DNA by electron microscopy. Carcinogenesis (Lond), 10 199-202. 1989. 

Prisyazhnoy, V.S.,M. Fusek, andY.B. Alakhov (1988). Synthesis of high-capacity immunoaffinity sorbents with oriented immobilized immunoglobulins or their Fab fragments for isolation of proteins. /. Chromatogr. 424 243-253. 

Fab fragments (M.W. 50,000) have several advantages over IgG (M.W. 150,000) for use as the immunotherapeutic reagent (Smith et al. 1979). The Fab fragments distribute more rapidly and extensively than intact IgG. In addition, they are catabolized and excreted earlier than intact IgG. A final important advantage is that the Fab fragments are less antigenic than the intact IgG. 

The affinity constant of the Fab fragments we produced for these studies (discussed below) was approximately 3.0xl09 M"1. This means that the Fab fragments would have about 138 to 750 times greater affinity for PCP than for the receptor. 

The affinity and cross-reactivity of the whole serum and Fab fragments were determined using equilibrium dialysis for the affinity determination and RIA for the cross-reactivity studies. The average intrinsic affinity constant (Ko) of the antibody (Nisonoff and Pressman 1958) changed very little throughout the... 

LD), in which flash vaporisation of the sample is induced, may be applied. Other techniques which permit detection of less-volatile chemical species are FD (with simultaneous desorption/ionisation of molecules), FAB (with the sample dissolved (dispersed) in a suitable liquid) and SIMS (based on bombardment of a solid surface with high-energy ions). LD-FUCR-MS is superior to FAB-MS for polymer/additive identification because it gives molecular ion fragmentation [83],... 

SIAB and sulfo-SIAB have been used to make a high-capacity RNA affinity column for the purification of human IRP1 and IRP2 (Allerson et al., 2003), to couple antibodies or Fab fragments to amine-modified microparticles (Harma et al., 2000), and in the attachment of oligonucleotides to surfaces for detection arrays (Adessi et al., 2000). 

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