Extraction terpene trilactone

Mauri, R Simonetti, R Gardana, C. Minoggio, M. Morazzoni, R Bombardelb, E. Rietta, R. Liquid chromatography/atmospheric pressure chemical ionization mass spectrometry of terpene trilactones in plasma of volunteers dosed with Ginkgo biloba L. extracts. Rapid Commun. Mass Spec., 2001, 15 929-934. 

Raising pressure at a fixed temperature improves the extraction efficiency due to an increased fluid density. In the extraction of bilobalide and ginkgolides, the maximum extraction efficiency was achieved when the density was at least 0.72 g/mL. Because the extraction of bilobalide and ginkgolides is improved at elevated temperature, high pressures are requited to olMain the minimum needed density. At the optimal temperature (100 °C), a density of 0.72 g/mL was obtained at 350 atm pressure. With the qitimized conditions (ethanol/acetic acid as a modifier, 100 °C and 330 atm), quantitative extraction of terpene trilactones from Ig sample of Ginkgo biloba was achieved with 20 min static and 20 min dynamic extraction times (two times of SFE). Under... 

The SFE technique was compared to the boiling liquid methods performed with the following solvents 1) acetone, 2) ethanol, 3) methanol, 4) water, and 5) water/methanol (70 30 vfv). The SFE method showed the highest extraction efficiency and reproducibility (RSD<5%, n = 3) for removing terpene trilactones from the ginkgo leaves in comparison with the solvent extraction methods. The total efficiencies for recovety of bilobalide and gingkolides (A, B, and C) with the solvent based methods were 60 91% relative to the SFE results (Table I). 

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