Extracellular and Membrane-Bound Proteases of B. subtilis

The Eprs of B. subtilis and related bacilli are also very relevant for commercial applications. In particular, these enzymes are employed in the manufacture of detergents, tanning of leather, management of industrial and household wastes, bioprocessing of X-ray or photographic films for the recovery of silver, protein hydrolysate preparation in the food industry, synthesis of aspartame, and other applications [102]. Recently, the fibrinolytic activity of Vpr was discovered. Accordingly, Vpr has the potential to work as a thrombolytic agent in medical applications [103, 104]. 

Notably, Eprs are not only beneficial for industrial applications. Their downside is that they represent major bottlenecks during the production of secreted 

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