Expression Systems Concluding Remarks

coli Economical, fast, easy, high yield, well characterized genetics, large number of cloning vectors Insolubility and misfolding of proteins, no glycosylation possible, difference in codon usage between prokaryotes and eukaryotes 

Mammalian cells Advanced post-translational modifications, signals for synthesis, processing and excretion are correctly recognized Time consuming, relatively low yield Complex regulatory system 

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Concluding this subsection, we remark that the expression (149) for the KS exchange energy of a mixed-state system is only approximate. The exact expression (204) is obtained in Sect. 5.4 and compared with the approximate one (149). The exact expression (236) for asymptotic is obtained in Sect. 5.6. 

Now let us see how this result is to be understood in terms of the DLVO theory. At first glance, it seems remarkable that any consistency at all can be found in tests as arbitrary as the CCC determination. It is not difficult, however, to show that these results are quite close to the values predicted in terms of the DLVO model for interacting blocks with flat faces. From an inspection of Figure 13.8, we concluded that the system at k = 108 m l would be stable with respect to coagulation, whereas the one at k = 3 108 m l would coagulate. Furthermore, we examined the energy barrier to draw these conclusions. Next we must ask how the qualitative criteria we used in discussing the curves can be translated into an analytical expression. 

Each section will express in detail features that can impart advanced properties to the polymer composites, with methods of surface modification, the central role of composites as sustained and controlled release systems (of drugs and other bioactive agents), and a part that reports on nanocomposites. The chapter concludes with general remarks and an insight of the likely future trends concerning the use of polymer composites for bone and cartilage tissue engineering. 

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