Expressed Sequence Tag database

Ulrich CM, Bigler J, Velicer CM et al. Searching expressed sequence tag databases discovery and confirmation of a common polymorphism in the thymidylate synthase gene. Cancer Epidemiol Biomarkers Prev 2000 9 1381-1385. 

Proteins fall into three classes when characterized with mass spectrometry proteins whose complete sequence is given in a database, proteins whose sequence is partially represented in expressed sequence tag databases, and proteins whose sequence is unknown. Proteins of the first class can be identified by peptide mass mapping in a very high through-... 

The second method relies on the partial interpretation of the spectra. A short amino acid stretch of one to three amino acids is read out. This sequence, together with its precise mass location in the peptide of a given mass, is used to identify the whole peptide in the database (Mann, 1996). Since the algorithms depend only on individual peptides, expressed sequence tag databases can be searched successfully. 

Ulrich, C. M., Bigler, J., Velicer, C. M., Greene, E. A., and Earin, F. M., Potter, J. D. (2000) Searching expressed sequence tag databases discovery and confirmation of a common polymorphism in the thymidylate synthase gene. Cancer Epidemiology, Biomarkers and Prevention. 9, 1381-1385. 

Identification of Novel Chemokines From Expressed Sequence Tag Databases... 

Wells, T. N. C. and Peitsch, M. C. (1997) The chemokine information source identification and chracterization of novel chemokines using the WorldWideWeb and expressed sequence tag databases. J. Leuk. Biol. 61, 545-550. 

Allikmets, R., B. Gerrard, A. Hutchinson and M. Dean. Characterization of the human ABC superfamily isolation and mapping of 21 new genes using the expressed sequence tags database. Hum. Mol. Genet. 5 1649-1655, 1996. 

WorldWideWeb and expressed sequence tag databases./oumoi of Leukocyte Biology, 61, 545-550. 

Novel Chemokines Identified in Expressed Sequence Tag Databases via Bioinformatics... 

---