Experimental RET in One Dimension

As found for eno gy tiansfa in monfaranes, useful in-fcvmation was obtained by exteninationof the steady-state data. Fm the D—A pair, the observed transfo  

Energy tr msfer in one dimension was also studied using the FD method. In this case the donor was Acridine Orange (AO) and the acceptor was a weakly fluorescent dye, Nile Blue (NB). Binding of NB to AO-DNA at a low dye/base-pair ratio resulted in significant quenching of the 

Hgure 15.1X FD intensiiy decay of AO-DNA wnth 0J)S8 NB/base pair. The lowerpanels show the retiduals fw the fits of the experimental data using the equations for RET in one ( ), two and three dimensions 

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