Experiment 22 Spectrophotometric Analysis of a Prepared Sample for Toluene

Experiment 22 Spectrophotometric Analysis of a Prepared Sample for Toluene 

Note The slightest contamination with water or other solvent will result in gross error. 

Prepare one 100-mL volumetric flask and five 25-mL volumetric flasks. These flasks should be clean and dry. They may be prepared by washing with soapy water and a brush, rinsing with water, removing the water with several rinses with acetone, and removing the acetone with several rinses with the alkane solvent to be used (e.g., hexane, heptane, or cyclohexane). 

Prepare 100 mL of a stock standard solution of toluene in an alkane solvent by pipetting 0.1 mL of toluene into the flask and diluting to the mark with the alkane. Use the 100-mL flask prepared in step 1. Shake well. This solution has a concentration of 0.870 g/L. 

Prepare four calibration standards with concentrations of 0.0435,0.0870,0.174, and 0.261 g/L in the alkane solvent by diluting the stock solution prepared in step 1. Use the 25-mL volumetric flasks prepared in step 1. 

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