Experiment 24 Fluorometric Analysis of a Prepared Sample for Riboflavin

Experiment 24 Fluorometric Analysis of a Prepared Sample for Riboflavin 

Note Riboflavin solutions are fight sensitive. Store them in the dark as you prepare them. 

Your instructor has prepared a 50 ppm riboflavin stock solution in 5% (by volume) acetic acid. Prepare a series of calibration standard solutions that are 0.2, 0.4, 0.6, 0.8, and 1.0 ppm from the 50 ppm stock solution and again use 5% acetic acid for the dilution. Use 25-mL volumetric flasks for these standards and shake well. 

Obtain the unknown from your instructor and again dilute with 5% acetic acid to the mark and 

Obtain fluorescence measurements (F) on all of your standards, the unknown, and control. Use 5% acetic acid for a blank. Your instructor will demonstrate the use of the instrument, and explain how it was set up to measure riboflavin. 

---