Examples of PLIMSTEX

PLIMSTEX was first demonstrated by using four model proteins rat intestinal fatty acid binding protein (I-FABP) interacting with potassium oleate (1 1), GDP-bound human protein (Ras- 

GDP) interacting with Mg (1 1), Ca -saturated porcine calmodulin (holo-CaM) interacting with the peptide melittin (1 1), and apo-CaM interacting with Ca (1 4) [18]. PLIMSTEX was able to provide accurate values for the four systems ranging from KP to 10 M . These early experiments also suggested that PLIMSTEX could be used for quick determination of binding stoichiometry and purity of the proteins [29]. 

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