Evolution yeast model

Dos Santos, A.M., Feuillat, M., Charpentier, C. (2000). Flor yeast metabolism in a model system similar to cellar ageing of the french Evolution of some by-products, nitrogen compounds and polysaccharides. Vitis, 39, 129-134. 

In 1966,1 also started a long-term project on the molecular genetics of yeast mitochondria. This model system provided important results as far as both the organization and the evolution of eukaryotic genomes are concerned. These findings will be briefly presented and discussed in Part 2 of this book. In the 1960 s we also performed a series of investigations on the physical chemistry of DNA (see, for instance, Froelich et al., 1963 Freund and Bernardi, 1963) and on transforming DNA from Haemophilus influenzae (Chevallier and Bernardi, 1965, 1968 Bernardi and Bach, 1968 Kopecka et al., 1973). 

Phosphofructokinase possesses two substrates, ATP and F6P, which it transforms into ADP and FBP. A complete model for this reaction should therefore take into account the evolution of these four metabolites. However, studies carried out in yeast indicate that the couple ATP-ADP plays a more important role than the couple F6P-FBP in the control of oscillations. Indeed, the addition of ADP ehcits an immediate phase shift of the oscillations (fig. 2.8) while the effect of FBP is much weaker (Hess Boiteux, 1968b Pye, 1969). The predominant regulation is thus exerted by ADP. In order to keep the model as simple as possible and to limit the number of variables to only two, which allows us to resort to the powerful tools of phase plane analysis, the situation in which an allosteric enzyme is activated by its unique reaction product is considered (fig. 2.10). This monosubstrate, product-activated. 

Gibson, A.C. (1982). Phylogenetic relationships of Pachycereeae. In Barker, J.S.F., Starmer, W.T. (eds.). Ecological Genetics and Evolution the Cactus-Yeast-Drosophila Model System, pp. 3-16. Sydney Academic Press. 

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