Evolution of Aldolase Antibodies In Vitro

Reactions and compounds for in vitro evolution of aldolase antibodies. 

Antibody-catalyzed reaction using cofactor primary amine. 

Cofactor-mediated Antibody-catalyzed Aldol and/or Retro-aldol Reactions 

An antibody that mimics threonine aldolase, which uses pyridoxal as the cofactor to catalyze the aldol reaction of glycine with aldehydes, has also been reported. Antibody 10H2 catalyzed the retro-aldol reaction of jd-hydroxy-a-amino acid in the presence of pyridoxal [55]. 

Class I aldolases function as Nature s most fundamental carbon-carbon bond forming enzymes. Designer catalysts - aldolase antibodies - that mimic the aldolases have been created by using a reaction-based selection strategy with 1,3-diketones. The covalent reaction mechanism is a fundamental part of the selection. Broad scope, enhanced catalytic activity, and defined chemical mechanism are three features of these aldolase antibodies that distinguish them from traditional antibody catalysts. The substrate specificities of aldolase antibodies are different from those of naturally existing 

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