Evaluation of Kinetic Parameters in Enzyme Reactions

In order to test the validity of the kinetic models expressed by Equations 3.28 and 3.31, and to evaluate the kinetic parameters and V ax experimental data with different concentrations of substrate are required. Several types of plots for this purpose have been proposed. 

Lineweaver-Burk Plot Rearrangement of the Michaelis-Menten equation (Equation 3.28) gives [4] 

CA/fp Versus Ca Plot Multiplication ofboth sides of Equation 3.32 by gives 

plot of against would give a straight line, from which the kinetic 

All of the above treatments are applicable only to uni-molecular irreversible enzyme reactions. In the case of more complicated reactions, additional kinetic parameters must be evaluated, but plots similar to that for Equation 3.32, giving straight lines, are often used to evaluate the kinetic parameters. 

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