Europium tetracycline

Europium tetracycline and GOx can be coadsorbed on hydrophilic sensor membranes as described in Sect. 2.4, adding up to ready-for-use glucose-sensitive luminescent foils. These can be implemented in microwell plate formats for a parallel screening of different samples [114]. Figure 18 shows eight adjacent wells of the plate spotted with differently concentrated glucose solutions in MOPS buffer, and the resulting calibration plot of the sensor. 

New europium probes have been presented that can indicate changes in hydrogen peroxide concentration and are applicable for time-resolved imaging. As H2O2 is a product of the activity of almost all oxidases, quantitative assays for different substrates, e.g glucose, or the determination of the activity of the respective oxidases can be carried out. On the other hand, peroxidases and catalases consume H2O2, which can also be monitored via the europium tetracycline probe and can therefore be used for the imaging of ELIS As performed in microwell plates. 

Imaging of Biomolecules Using the Europium(III) Tetracycline Probe ... 

Fig. 20 Mechanism of a sandwich ELISA for the detection of bovine IgG using peroxidase (POx)-labeled anti-IgG and the europium(III) tetracycline system as fluorescent reagent (top). Schematic of the microwell surface showing the assembly of the sandwich assay (bottom)...

As might be expected, some of the high-volume inorganics (e.g., chlorine and sodium hydroxide) have the lowest prices, and low-volume chemicals like the drug tetracycline hydrate and the rare earth chemical europium in oil, have the high prices of 0.90 and 71.50 per gram, respectively. 

Table 3 Luminescent probes based on europium(in) tetracycline complexes ligand structures and corresponding analytes...

Fig. 5 L Luminescence decay profiles of the 615 nm emission line of europium(III) tetracycline 11 in absence (EuTc) and presence of H2O2 (EuTcHP) as recorded by time-correlated single photon counting. Right absorption and emission spectra of EuTc at pH 6.9 in presence and absence of H2O2 (/lexc = 405 nm), displaying the Fj transitions (see. Fig. 1)...

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