Eukaryotes genetic organization

Before we study how genomic libraries are made we first need to understand the differences between the genetic organization in eukaryotic and prokaryotic cells. Bacterial genes are uninterrupted sequences of... 

Before we move to these subjects in the following sections, it should be stressed that the organization of the mitochondrial genome of yeast points to the fact that in eukaryotic genetic systems, where so much of the DNA is non-coding, there is a real need for a molecular approach, because approaches based on classical genetics or on the study of gene products suffer from serious intrinsic limitations and are unable to provide an overall... 

Despite the differences in nuclear structures between prokaryotes and eukaryotes, the genetic code, i.e. the combination of bases which does for a particular amino acid in the process of protein synthesis, is the same as it is in all living organisms. 

From a genetical point of view, Saccharomyces cerevisiae is an ideal organism which may be considered the Escherichia coli of eukaryotic cells [4,5]. This is true in particular for the study of metabolic regulation and for that of membrane transport [6]. Finally, the astonishing resemblance between many yeast proteins and certain mammalian-cell proteins has seriously broadened the scope of interest. Although a few reports have appeared on amino acid transport in some other yeasts, most investigations in this field have used strains of Saccharomyces cerevisiae. 

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