Eukaryotes amino acid composition-based

This enzyme has little specificity for the base composition of its substrates, utilising both ribonucleotides and deoxyribonucleotides. NDPKs have been cloned and/or purified from numerous sources including mammals, plants, insects, lower eukaryotes and prokaryotes. The primary amino acid sequences of NDPKs show high levels of sequence homology with the functional monomer being around 17 kDa. However, the enzyme appears to function in vivo as an oligomer, this being hexameric in eukaryotes and... 

DNA polymerases perform essential roles in the replication and repair of genetic material. The DNA polymerases of several mesophiles have been extensively characterized, and the structural, biochemical, and kinetic properties of Escherichia coli, yeast, mammalian, and bacteriophage DNA polymerases have been the subject of numerous reviews. " Certain enzymes complement DNA polymerase activity with 3 - -5 -exonuclease (proofreading) activity and/or 5 - 3 -exonuclease activity, which typically reside in separate structural domains on the same polypeptide. To orchestrate DNA replication and repair, both eubacteria and eukaryotes possess multiple DNA polymerases, each with distinct properties, subunit compositions, and physiological roles. In vivo, DNA polymerases require the assistance of numerous accessory proteins to replicate and repair the genome (reviewed ). Based on amino acid sequence homology to E. coli enzymes, DNA polymerases have been classified into at least three distinct families Family A (Pol I-like), Family B (Pol Il-like), and Family C (Pol Ill-like). ... 

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