Ethylenediaminetetraacetic acid titrations separations

The determination of calcium in meat products, especially in mechanically recovered (or separated) meat (MRM/MSM), is an important estimation of the amount of bone material included in the meat. The AOAC method describes an acid digestion of the sample and reaction of an aliquot of the filtered digest with excess ethylenediaminetetraacetic acid (EDTA) under alkaline conditions to form a chelated complex with calcium ion. The excess EDTA is then titrated with calcium carbonate using hydroxynaph-thol blue as indicator. 

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