9-Ethyladenine imprinted polymers

Spivak D, Gilmore MA, Shea KJ. Evaluation of binding and origins of specificity of 9-ethyladenine imprinted polymers. J Am Chem Soc 1997 119 4388-4393. 

Table 4-1 Capacity factors (fc) of nucleotide analogs on HPLC columns packed with 9-ethyladenine-imprinted polymer of methacrylic acid and benzoic acid-imprinted polymer [1 ]a...

A nudeobase derivative, 9-ethyladenine-imprinted polymer capable of spectroscopic change based upon molecular recognition has also been prepared using the zinc(II) porphyrin-based functional monomer [25]. 

Shea et al. have prepared an imprinted polymer (a matrix of polymethacrylate) using 9-ethyladenine (9-EA) as a template (Figure 62) [85], They have shown that the average association constant for this substrate is of the order of... 

A nucleotide base-imprinted polymer membrane has been reported in which methacrylic acid was used as a functional monomer for the imprinting of an adenine derivative, 9-ethyladenine [41], A free-standing film was prepared by polymerizing a DMF solution containing methacrylic acid and ethylene glycol dimethacrylate on a silanized glass slide at 65-70 °C under nitrogen atmosphere. 

Table 8-4 Retention properties of the 9-ethyladenine(9-EA)-imprinted and non-imprinted polymers obtained by using two kinds of functional monomers...

Huang, Y.C., Lin, C.C., and Liu, C.Y., Preparation and evaluation of molecularly imprinted polymers based on 9-ethyladenine for the recognition of nucleotide bases in capillary electrochromatography. Electrophoresis, 25, 554, 2004. 

Matsui, J. Higashi, M. Takeuchi, T. Molecularly imprinted polymer as 9-ethyladenine receptor having a porphyrin-based recognition center. J. Am. Chem. Soc. 2000, 122,... 

Takeuchi and co-workers [45] have studied the preparation of imprinted polymers using metalloporphyrin as the recognition center. In this study, 9-ethyladenine (9-EA) (Fig. 8, 32) was used as a model compound. It was found that the use of two functional monomers MAA and a Zn-porphyrin-based monomer (Fig. 8, 33) significantly increased the affinity of the resulting polymer for the template molecule. Upon addition of 9-EA to the imprinted polymer significant fluorescence intensity... 

Metalloporphyrins have been used for the development of MIP for detection of a 9-ethyladenine nucleobase derivative [35]. With the increase of the template concentration, the bulk polymer exhibited a red shift in the absorbance spectra. This shift allowed for the quantitative detection of the template, which showed formation of a 1 1 monomer-template complex. Moreover, cinchonidine was imprinted using metalloporphyrin and MAA as the functional monomers [40]. The resulting MIP provided high selectivity against a diastereomer of the... 

A fluorescent MIP chemosensor for determination of 9-ethyladenine was fabricated [56]. It contained porphyrin as a luminescent functional monomer. The interaction of 9-ethyladenine with the porphyrin quenched the MIP luminescence at 605 nm when excited at 423 nm. The polymer was sensitive to 9-ethyladenine in the range of 0.01-0.1 mM however, it was already saturated at 0.15 mM. The same researchers used vinyl-substituted zinc(II) porphyrin and methacrylic acid as functional monomers for imprinting of (-)-cinchonidine [57]. The MIP luminescence, when excited at 404 nm, was significantly quenched at 604 nm upon binding of (-)-cinchonidine, even in the low concentration range of 0.01-2 mM. 

Recently, Takeuchi and coworkers [37] reported the use of molecular imprinting for constructing a highly specific porphyrin-based receptor site. 9-Ethyladenine [37] was chosen as the imprint molecule. Two different functional monomers were utilized to bind 58 during the polymerization process, methacrylic acid (MAA) and a polymerizable zinc-porphorin derivative, 59 (P-53), as shown in Fig. 20. Reference polymers imprinted with 56 were fabricated using either 59 or MAA (P-54 and P-55, respectively) and corresponding nonimprinted, blank polymers were prepared using MAA and 59, MAA, or 57 as functional monomers to form polymers P-56, P-57, and P-58, respectively (Fig. 21). 

Table 10 Capacity Factors of Polymers Imprinted with 9-Ethyladenine Employing Chloroform and Acetonitrile as Porogen and Mobile Phase (Adapted from Ref. 17)...

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