Ethanol stimulation of poly

Mechanism of Ethanol Stimulation of Poly(ADP-Ribose) Synthetase... 

The ester protection technique has also been used in the preparation of amino acid derivatives of polyphosphazenes (70, 71). For example, eAyl glycinate reacts with poly(dichlorophosphazene) to link the amino function to e phosphorus atoms of the backbone. Subs uent exposure of the protected polymer to water in a biological environment leads to hydrolysis of the ester function and breakdown of the polymer to ethanol, glycine, phosphate, and ammonia. This has been used both for the controlled delivery of drugs and for stimulating the regrowth of bone in tissue engineering experiments (72). 

Fujii and Villee (124) reported that RNA, isolated by cold phenol extraction from the vesicular glands of adult rats or immature testosterone-treated animals and then treated with DNase and pro-nase, can stimulate growth and incorporation of labeled leucine into protein when the RNA is instilled into the central lumen of the seminal vesicles of immature or postpubertal rats. Treatment of the RNA preparations with RNase abolished these effects. RNA from seminal vesicle of an untreated immature or orchiectomized adult rat was ineffective. In more recent experiments from the same laboratory (125), the vesicular gland RNA from sexually mature rats was extracted with cold phenol, treated with DNase and ethanol and ether, and then separated into poly(A) RNA and poly(A) RNA fractions by chromatography on Sepharose 4B-inidylic acid. When the desalted RNA fractions were instilled into seminal vesicles of castrated rats, only the poly(A) RNA... 

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