Estrogen Receptors in Prostate Tissue

To localize ERp on paraffin sections, 65-kDa antirat ER antibodies are used (Upstate Biotechnology, Lake Placid, NY). This antibody is obtained by immunizing rabbits with synthetic peptides representing the N-terminal amino acid residues 46-63 of human ER(3. The deparaffinized sections on slides are placed in the Target Retrieval Solution (pH 6.1) 

To localize ER[3 in frozen sections, antigen retrieval with heating is not required. 210-180-C050 antibodies (Alexis Corporation, Nottingham, UK) are obtained by immunizing rabbits with synthetic peptides representing the C-terminal amino acid residues 467-485 of human estrogen. 

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