Esters analysis

L. M. Mallory and G. S. Sayler, Application of FAME (fatty acid methyl ester) analysis in the numerical taxonomic determination of bacterial guild structure, Mi-croh. Ecol. t0 l%2 (1984). 

The optical purity and stereochemistry was established by conversion to the known A-BOC protected proline and by comparison of spectral data and rotation values161. Additionally, the optical purity was established by HPLC analysis of the 3,5-dinitrobenzoates on a Pirkle column and by Mosher ester analysis. 

Organic esters, 10 497-526. See also Esters analysis, specifications, and standards for, 10 508... 

Gordon, M.H. and Miller, L.A.D. (1997) Development of steryl ester analysis for the detection of admixtures of vegetable oils. J. Amer. Oil Chem. Soc., 74, 505-510. 

The analysis of alkyd resins was a subject of interest due to many practical applications of these materials [46-48]. Similar to other ester analysis, the pyrolysis with hydrolysis/alkylatlon, for example in the presence of TMAH, can be applied successfully for those materials [49, 50). Pyrolysis alone is basically similar to that of other esters of aromatic diacids. 

Gas Chromatography for Fatty Acid Methyl Ester Analysis... 

In Chapter 8 a leading expert in the detection of adulteration of oils and fats, Rossell, explains the economic advantages which tempt unscrupulous dealers to add cheap oils to high-value oils such as olive oil. He then describes a technique which he and his coworkers at Bristol have pioneered where stable carbon isotope ratio (SCIR) measurement has simplified the task of detecting adulteration. He compares sterol and fatty acid methyl ester analysis with SCIR and shows how clearly the latter detects maize oil. 

Fatty Acid Ester Analysis by Ag-HPLC. Ag-HPLC analysis of CLA isomers as FAME continues to be the single largest application for this technology. An excellent example is illustrated in Figure 3.3, the only figure in this chapter duplicated... 

Fig. 3.22. Autoxidation of linoleic acid methyl ester. Analysis of primary products (after reduction of the hydroperoxy group) by HPLC (according to Chan and Levett, 1977). 1 13-Hydroxy-cis-9, trans-ll-octadecadienoic acid methyl ester, 2 13-hydroxytrans-9,trans-ll-octadecadienoic acid methyl ester, 3 9-hydroxy-trans-lO, cis-12-octadecadienoic acid methyl ester, 4 9-hydroxy-trans-lO, trans-12-octa-decadienoic acid methyl ester...

Esters of retinol with long-chain fatty acids are a major storage form of vitamin A, espeeially in liver but also in other tissues. In some cases, it may be convenient to saponify the sample and assay total vitamin A as retinol in other cases, more information can be gained by analyzing the intact retinyl esters. Because all retinyl esters and retinol have identical molar absorptivity in a given solvent (123), retinyl palmitate can be used as a single quantitative standard for retinyl ester analysis if peak area (but not peak height) is used. 

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