Ergot alkaloids analytical methods

Flieger, M. et al. (1997). Ergot alkaloids — sources, structures and analytical methods. Folia Microbiol. 42(1), 3-29. Glass, J. et al. (2002). Streptococcus pneumoniae as a genomics platform for broad spectrum antibiotic discovery. Curr. Opin. Microbiol. 5(3), 338—342. 

Flieger M, Wurst M, Shelby R Ergot alkaloids — sources, stmctures and analytical methods. Folia Microbiol 1997 42 3-30. 

A very detailed review of the HPLC methods has been carried out [7]. The author has described the stationary phase, the mobile phase, flow rate, and detector system used by researchers since 1973. We would like to describe the other analytical methods such as the capillary electrophoresis, flow injection analysis and two-dimensional fluorescence spectroscopy which have foimd applications in ergot alkaloid research. 

The separation of ergot alkaloids by h.p.l.c. is recommended as an analytical procedure, since it is rapid, accurate, and reproducible the method was illus-... 

Analytical methods for the detection and analysis of ergot alkaloids by high-performance liquid chromatography (HPLC) and tandem mass spectrometry (Yates and Powell, 1988), and both the H- and C-NMR (nuclear magnetic resonance) spectra for these compounds have been reviewed (Crabb, 1982). The results of feeding studies leading to the formation of ergot alkaloids have been tabulated (Leete, 1983). 

Sulyok et al. [113] reported the first validated method for the determination of 39 mycotoxins in wheat and maize by liquid chromatography with electrospray ionization-triple quadmpole mass spectrometry (LC/ESI-MS/MS) without the need for any cleanup. The 39 analytes included A and B trichothecenes (including deoxynivalenol-3-glucoside), ZEN and related derivatives, fumonisins, enniatins, ergot alkaloids, ochratoxins, aflatoxins, and moniliformin, and six trichothecene mycotoxins (NIV, DON, fusarenon-X, 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, and T-2 toxin). 

Analytical methods have so far concentrated mainly on ergot alkaloids from C. purpurea and few data are available for alkaloids from other Claviceps spp. infecting grain. Only a small number of aU ergot alkaloids are well characterized, and several unknown compounds including bound forms might contribute to the health hazard posed by food and feed contamination. 

Komarova EL, Tolkachev ON (2001) The chemistry of peptide alkaloids. Part 2. Analytical methods fm determining ergot alkaloids. Pharmacent Chtan J 35 542-549... 

Flieger, M., Wurst, M. and Shelby, R. (1997) Ergot alkaloids—sources, structures and analytical methods. Folia Microbiol., 42, 3-30. 

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