Enzymes, methanotrophic isolates

Table I. Methane Monooxygenase Enzymes in Characterized Methanotrophic Isolates...

Vinyl chloride by methanotroph mixed culture C. Cell-free extracts or isolated enzymes 60 /tM 2 x 10 3... 

The enzyme system MMO can be isolated from methanotrophic bacteria, which use methane as their only source of carbon and energy. MMO catalyzes the first step in the metabolic pathway of the organism, eq. 1. Research in our... 

Availability of methane determines quality of methanotrophic community. Soils incubated in the atmosphere enriched with methane (10% vol.) showed high capacity for methane oxidation but they do not show the ability to oxidize methane at atmospheric (ambient) concentration (Walkiewicz et al. 2012). This phenomenon can result from the presence of the type II methanotrophs in soils. Those methanotrophs have methanotrophic maximum activity and a low affinity to CH (high value of the Michaelis constant Kj ) (Bender Conrad, 1993). Both the parameters are determined on the base of Michaelis-Menten equation. K constant represents the substrate concentration at which the rate of an enzyme-catalysed reaction is half of the maximum value. The type II bacteria are isolated from the environments rich in methane (>1%) and poor in oxygen (about 1%) (Hanson Hanson, 1996), such as bog peat or landfill covers. They are less sensitive to the environmental changes than the type I bacteria which has a low value of and high affinity to methane (Henckel et al. 2000) proved by the low value... 

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