Enzyme gold-labeled

Berg R. H., G. W. Erdos, M. Gritzali and R. D. Brown. (1988). Enzyme-gold affinity labeling of cellulose. Journal of Electron Microscopy Techniques 8 371-379. 

Brown-rot fungus (isolate MAD-698) was grown on sterile 12 mm glass slips placed on the surface of 2% (w/v) malt agar removed when about 50% covered were localized using colloidal gold labeled monoclonal antibodies to the B-l, 4-xylanase fraction of P. placenta-, enzymes were localized on the hyphal surface... 

In this approach double-stranded oligonucleotide PCR amplicons are labeled with a hapten on one end and another different hapten on the other end during PC R. The transducer surface (gold or carbon screen-printed chips) is treated with protein based anti-haptens that capture one of the hapten label on the PCR amplicons. The second hapten label on the PCR amplicons will bind to a specific anti-hapten-conjugated HRP or AP reporter enzyme to labeled PCR amplicons. Addition of the enzyme-specific redox substrate and application of a fixed potential between working and reference... 

The choice of label will depend on the application envisaged. Enzymes are widely applicable they are used in assays, such as ELISAs, and for detection of antigen blotted or dotted on membranes or embedded in tissue sections. Enzyme labels have also be used for the location of antigen in electron microscope sections but gold labelled antibodies (Chapter 11) are now extensively employed for this purpose. Antibodies labelled with a fluorescent molecule are used in assays and for the detection of antigens in tissue sections and also for flow cytometiy and fluorescence-activated cell sorting. 

Hu, R., Wen, W., Wang, Q., Xiong, H., Zhang, X., Gu, H., Wang, S., 2014. Novel electrochemical aptamer biosensor based on an enzyme-gold nanoparticle dual label for the ultrasensitive detection of epithelial tumour marker MUC1. Biosens. Bioelectron. 53, 384—389. 

Figure 4. Transmission electron micrograph showing tuber tissue labelled with protein A-gold. Localization of PL3 enzyme is indicated by arrow-heads. S= Starch grain CW= Cell wall Bar= SOOnm. x 20 000.

Jemmerson, R., and Agre, M. (1987) Monoclonal antibodies to different epitopes on a cellsurface enzyme, human placental alkaline phosphatase, effect different patterns of labeling with protein A-colloidal gold. J. Histochem. Cytochem. 35, 1277-1284. 

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