Enzyme mediated microanalysis

The same reaction was recently proposed to detect creatine kinase (CK), an enzyme of high clinical significance in relation to the investigation of skeletal muscle disease and the diagnosis of myocardial infarct or cerebrovascular accidents. As ATP is a reaction product obtained from the reaction of ADP with creatine phosphate catalyzed by CK, this enzyme can be indirectly measured by the CL intensity read from the subsequent reaction of ATP with luciferin. Using the technique of electrophoretically mediated microanalysis (EMMA), it is possible to detect the enzyme using nanoliter volumes of biological sample with an improved speed and simplicity with respect to a conventional colorimetric method [100],... 

Electrogenerated chemiluminescence Enzyme-linked immunosorbent assay Electrophoretically-mediated microanalysis Electroosmotic flow Eosine Y... 

Most applications of native fluorescence detection involve the direct detection of a fluorescent analyte of interest. However, native fluorescence can also be used indirectly to detect the presence of nonfluorescent species. For example, the presence of a nonfluorescent enzyme can be detected based on its conversion of substrate into natively fluorescent products. This is often used in conjunction with electrophoretically mediated microanalysis, in which differences in buffer mobilities enable on-column mixing and reaction of enzymes with substrates. One such study exploited the intrinsic fluorescence of nicotinamide adenine dinucleotide (NADH) to investigate differences in the reactivity of individual molecules of lactate dehydrogenase. 

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