Enzyme-linked immunosorbent inhibitors

The CML is the most characterized AGE and is referred to as a glycoxidation product. The inhibitory effects of C-glycosylflavones on the CML formation were tested by enzyme-linked immunosorbent assay in kidney diabetic subjects. The results showed that the percent inhibition was about 53% for chrysoeriol 6-C-boivinosyl 7-0-glucoside, 64% for chrysoeriol 6-C-boivinosyl, 80%i chrysoeriol 6-C-fucosyl, and only 2% for 4"-OH-3 -methox-ymaysin versus 60%i for the standard glycation inhibitor, aminoguanidine. 

Many types of assay are available to be used in HTS protocols to identify inhibitors of PPIs, but a competition assay, in which inhibition of complex formation is measured, is most common. Fluorescence polarization (FPA), fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assays (ELISA), and other assay formats have been used. The interacting proteins can be used in their full-length forms though, more frequently only the interacting domains are employed, and if possible the excised interacting peptide is usually preferred. 

ELISA enzyme-linked immunosorbent assay ICAD inhibitor of caspase-activates Dnase... 

Sweep, C. G. J., Geurts-Moespot, J., Grebenschikov, N., et al.. External quality assessment of trans-european multicenter antigen determination (enzyme-linked immunosorbent assay) or urokinase plasminogen activator (uPA) and its type-1 inhibitor (PAI-1) in human breast cancer extracts. Br. J. Cancer 78, 1434-1441 (1998). 

Fig. 6. Effects of cyclooxygenase inhibitors on lipopolysaccharide (LPS)-induced tumor necrosis-factor (TNF)-a production. THP-1 cells pretreated with indomethacin (5 pM), niflumic acid (2 pM) or aspirin (2 mM) were stimulated with EPS for 6 h. TNF-a levels in cell culture supernatant were determined by enzyme-linked immunosorbent assay. The amount of TNF-a produced in the control LPS-stimulated cells is set as 100%. Values are means SEM, n = 3. The treated groups did not differ from the control group. 

Levels of cytoplasmic oligonucleosomal DNA (cell death detection enzyme-linked immunosorbent assay) were significantly enhanced for chryso-tUe (3-25 pg/ml) and crocidolite (25-75 pg/ml) in a dose-dependent manner, a process that was inhi-bitable by 10 pM Z-Val-Ala-Asp fluoromethyl ketone, an interleukin-converting enzyme inhibitor (Hamilton et al. 1996). 

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