Enzyme kinetics biomolecular reactions

Because of the high sensitivity and the small reaction vessel, these instruments are very suitable for biomolecular and pharmaceutical research purposes. Typical applications are the measuring of protein and drug binding interactions, characterization of enzyme kinetics, and other biochemical problems. 

Some dye molecules have a different x>Ka in the electronically excited state, so that they release or take up H ions when exposed to a flash of light. This can be used to bring about rapid pH changes in solution. Such pH-jump experiments can be used to follow the kinetics of biomolecular processes. Other kinds of photochemical reactions can be used to overcome mixing problems in rapid kinetic experiments. One example of this is the use of caged ATP compounds that only become available for enzyme reaction, for example, when exposed to an intense light flash. ... 

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