Enzyme Kinetics and Their Reactions

The enzyme [E) binds a substrate (S) and produces a product [P]. Enzyme kinetics is the investigation of how enzymes bind substrates and turn them into products. An enzymatic reaction occurs in two stages. In the first stage, the substrate binds reversibly to the enzyme, forming the enzyme-substrate complex. This is called the Michaelis complex. The mechanism of single-substrate enzyme catalytic reaction is shown in Fig. 4.2. 

Enzymes can catalyze up to several million reactions per second. Enzyme rates depend on solution conditions and substrate concentration (Jayam et al., 2005]. The maximum speed of an enzymatic reaction is based on the substrate concentration until a constant rate of product formation. This is shown in Fig. 4.3 for indicating the saturation curve. Michaelis-Menten constant (/fm) is the substrate concentration required for an enzyme to reach 

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