Enzyme immunoassay systems

The general method utilized to prepare E5-Ab solutions obviates the need for stocking large numbers of reagents which would be necessary if different activation methods were used for each antibody. A number of specific antibodies immobilized by this process have shown response similar to that of the same antibodies when adsorbed as immune complexes in the Stratus system. In addition, the dendrimer-coupled antibodies have shown dramatic improvements in sensitivity, flexibility and precision for the enzyme immunoassay system. Feasibility demonstration of an assay for DNA probes is a prelude to what can possibly be achieved with these dendrimer-based reagents. 

Huber, S.J. (1985). Improved solid-phase enzyme immunoassay systems in the ppt range for atrazine in fresh water. Chemosphere, 14 1795-1803. 

Blais, B.W., Gaudereault, M., and Phillippe, L.M. 2003. Multiplex enzyme immunoassay system for the simultaneous detection of multiple allergens in foods. Food Control 14 43 17. 

ST32 Stahlschmidt, G.E., Smith, S.H., Miller, R.E. and Landt, M.L. (1987). The Stratus fluorometric enzyme immunoassay system evaluated for the determination of total thyroxine. Clin. Chem. 33, 883, Abstr. 17. 

Comparison of the ES 600 automated enzyme immunoassay system with Stratus immunofluorometry and manual radioimmunoassay. Clin. Chem. 33, 947, Abstr. 327. 

ST49 Banfi, G., Racca, S., Daverio, R., Murone, M. and Bonini, P.A. (1989). Free thyroxin estimation by Stratus fluorometric enzyme immunoassay system. Biochim. Clin. 13, Suppl. 1/ 8, 133, Abstr. W 40. 

STN14 Moore, J.J., Sax, S.M. and Nadkami, S.R. (1991). Prolactin analysis utilizing Stratus fluorometric enzyme immunoassay system, Clin. Chem. 37,936, Abstr. 127. 

Meyerhoff ME, Duan C, Meuse M, Novel nonseparation sandwich-type electrochemical enzyme immunoassay system for detecting marker proteins in undilute blood, Clin Chem 1995 41 1378-84. 

AJ McNally, et al. A convenient enzyme immunoassay system for detection of drugs of abuse—Cannabinoids in urine. Clin Chem 33 972, 1987. 

S.J. Huber, Improved Solid-phase Enzyme Immunoassay Systems in the ppt Range for Atrazine in Fresh Water , Chemosphere, 14,1795-1803 (1985). 

Miyai K (1981) Enzyme immunoassay system - schematic representation. In Ishikawa E, Kawai T, and Miyai K (eds.) Enzyme Immunoassay, pp. 123-135. Tokyo Igaku-Shoin. 

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