Enzyme, cleft

Kemp s Acid Enzyme-Cleft and Self-Replication Models... 

Kemp s Acid Enzyme-Cleft and Self-Replication Models 347 5.2.1 Enzyme-Cleft Models with Convergent Functional Croups... 

Enzyme-Cleft Models with Convergent Functional Croups... 

Global minimum conformations within the enzyme clefts Folded conformation with the phenyl of moiety A in close proximity to the thiazo-... 

Another interesting application of a structurally ill-defined assembly is the first efficient hydrolysis of an amide at room temperature and pH 8. When the cationic amide 15 (2 x 10 M) is mixed with anionic palmitate (2 x 10 M)at pH 8, an undefined molecular cluster is formed in which the water-stable amide (t./, > 1 yr) rapidly hydrolyzes = 3.1 min). The long-time contact of reactants, a state thought to be essential for enzyme-like reactions, can obviously not only be enforced by neighbouring group effects in rigid molecules or in complexes between enzyme clefts and substrates. It may also occur in very simple micellar-like clusters of extremely low cmc. 

Biochemical reactions take place in the hydrophobic centers of membranes or enzyme clefts. Aqueous compartments of biological cells are usually taboo media for the formation of small molecular assemblies or covalent compounds. There is, however, one notable exception, namely the formation of inclusion... 

We will give a detailed description of recent insights obtained especially for the widely studied hexakinase enzyme, which catalyzes the phosphorylation of glucose. The phosphorylation of glucose by hexakinase has been modeled in detail by simulating the reaction events that can occur in the enzyme cleft PI. The free hexakinase enzyme molecule is... 

The shape of the enzyme cleft is clearly more complex than for the A9 desaturase and involves polar sites capable of weakly interacting with existing double bonds in the substrate at positions 9-10, 12 13, 13-14 and 15-16. A greater range of possible substrates needs to be tested before the shape and dimensions of the cleft can be more accurately defined. 

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