Enzyme and Microbial Kinetics

It should be recognized that the analysis of enzyme kinetics grew up in a related but different branch of science largely biological—than the surface reaction kinetics we have been discussing, which comes largely from physical chemistry. As a result, one will see that the results are strikingly similar to many of those already seen, but the names and nomenclature are completely different. 

Thus we end up quickly with a familiar-looking form for the rate equation 

In the form, and with the nomenclature most often used in the biochemical literature, this is 

The procedure used in deriving the Michaelis-Menton equation is readily generalized. Equation (3-73) supposes that the decomposition of complex X is reversible, but suppose that it is not that is 

It is apparent that we must define more than one Michaelis constant, and more than one value of V. Considering only the forward rate (P = 0), 

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Enzyme and microbial kinetics involve the study of reaction rates and the variables that affect these rates. It is a topic, that is critical for the analysis of enzyme and microbial reacting systems. The rate of a biochemical reaction can be described in many different ways. The most commonly used definition is similar to that employed for traditional reactors. It involves the time change in the amount of one of the components participating in the reaction or of one of the products of the reaction this rate is also based on some arbitrary factor related to the system size or geometry, such as volume, mass or interfacial area. In the case of immobilized enzyme catalyzed reactions, it is common to express the rate per unit mass or per unit volume of the catalyst. 

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