Enzymatic synthesis Mucor miehei

The biotechnological synthesis of lactones has reached a high standard. Besides microbial production, lactones can also be enzymatically produced. For instance, a lipase-catalysed intramolecular transesterification of 4-hydroxy-carboxylic esters leads enantioselectively (ee>80%) to (S)-y-lactones the chain length may vary from C5 to Cl 1 [13]. y-Butyrolactone can be produced in that way with lipase from Mucor miehei [30]. 

In this work we have shown that it is possible to use an ezyme to catalyze the polycondensatioh reaction to form polyamides. The large number of polyamides that have been made indicate diat lipases from Candida antarctica and Mucor miehei are rather nonspecific and can be used generally for polyamide synthesis. In a particular example, a water-soluble polyamide has been produced from dimethyl adipate and ethylene triamine via this enzyme-catalyzed reaction at 50-110°C. The enzymatic polymerization is easy to do and... 

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