Enzymatic reactions in liposomes

Such a research project is a complex enterprise and it may be useful to divide up the road map to the minimal cell into different milestones of increasing complexity. The first one, which is already under control in several laboratories, is to carry out and optimize complex enzymatic reactions in liposomes - such as the polymerase chain reaction, the biosynthesis of RNA and DNA, the condensation of amino acids, etc. 

Of the various strategies for conducting enzymatic reactions in liposomes involving nucleic acids, three strategies are discussed here because these strategies have also been applied and their results published in the literature. 

Oberholzer, T. et al.. Enzymatic reactions in liposomes using the detergent-induced loading method, Biochim. Biophys. Acta, 1416, 57-68, 1990. 

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