Enzymatic hydrolysis computer simulations

The principal features of a mathematical model described for the enzymatic hydrolysis and fermentation of cellulose by Trichoderma reesei are the assumption of two forms of cellulose (crystalline and amorphous), two sugars (cellobiose and D-glucose), and two enzymes (cellulase and j3-D-glucosidase). An inducer-repressor-messenger RNA mechanism is used to predict enzyme formation, and pH effects are included. The model consists of 12 ordinary differential equations for 12 state variables and contains 38 parameters. The parameters were estimated from four sets of experimental data by optimization. The results appear satisfactory, and the computer programs permit simulation of a variety of system changes. 

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