Enzymatic degradation products, analysis

Fig. 4. (right) Polyacrylamide gel analysis of degradation products of ribose terminus-labeled poly(ADP-ribose) with different chain sizes. Three groups (referred to as L, M, and S, Fig. 3) of ribose terminus-labeled poly(P P]ADP-ribose) were prepared by electroelution of polyacrylamide gel pieces horn (Afferent areas. Enzymatic degradation products of these polymers were analyzed as described in the legend to Fig. 3. A, B, and C stand for polymers to S, M, and L, respectively (Reprinted with permission from ref. 8).

No other cofactors are required for enzymatic catalysts of this reaction. Amino acid analysis on isolated isoenzymes I and III indicate that the lower molecular weight monomers from I are not proteolytic degradation products of the monomers from III [68], When dimers of I (a ) are mixed with dimers of III ()8y8) an equilibrium concentration of II (afi) is formed. 

Elashvili. I. (2Enzymatic analysis of nerve agents and their degradation products. Paper pte.scntcd at the 24th Army Science Conference Tran.sformattonal Science and Technology for Current and Future Force, KP-13. Available at www,a.sc2004.com/ 23rd/summaries/k/kp-13.pdf. 

Pham, R, Dupont, I., Roy, D., Lapointe, G., Cerning, J. (2000). Production of exopolysaccharide by Lactobacillus rhamnosus R and analysis of its enzymatic degradation during prolonged fermentation. Applied and Environmental Microbiology, 66(6), 2302-2310. 

TLC has proved suitable for analysis of the products of enzymatic degradation of nucleotide coenzymes [38, 72] and of soluble ribonucleic acids [3] it has been demonstrated that enzymatic reactions can be carried out on layers of PEI-cellulose [72]. The biosynthesis of nucleosides [110], ribonucleoside- and deoxyribonucleoside triphosphates [52], diribonucleoside di-, tri- and tetraphosphates [76, 108, 111] and polynucleotides [62] has been followed with the help of TLC. 

---