Enzymatic degradation poly films

Ren KF, li 1, Shen 1C (2006) Construction and enzymatic degradation of multilayered poly-L-lysine/DNA films. Biomaterials 27 1152-1159... 

Although the QCM has been used to quantitate the mechanisms of enzymatic reactions in some instances, more often it has been used to determine the overall rates and characteristics of products formed in these reactions. For example, multistep processes have been studied that are involved in the enzymatic degradation of polymeric films formed from poly(L-lactide) [61] and poly(3-hydroxybutyrate) [60]. In QCM studies at a high resonance frequency, 27 MHz, DNA polymerase was studied using immobilized DNA to obtain rate constants for three individual steps in the mechanism of this enzymatic reaction ( [65] Chap. 10 in this volume). 

ZHA 11] Zhang J., Kasuya K., Hikima T., et al, Mechanical properties, structure analysis and enzymatic degradation of uniaxiaUy cold-drawn films of poly[(R)-3-hydroxybutyrate-co-4-hydroxybutyrate] . Polymer Degradation and Stability, vol. 96, no. 12, pp. 2130-2138, 2011. 

Abe H, Doi Y (1996) Enzymatic and environmental degradation of racemic poly(3-hydroxybutyric acid)s with different stereoregularities. Macromolecules 29 8683-8688 Abe H, Doi Y, Aoki H, Akehata T (1998) Solid-state structures and enzymatic degradabilities for melt-crystallized films of copolymers of (f )-3-hydroxybutyiic acid with different hydroxyal-kanoic acids. Macromolecules 31 1791-1797... 

Yamashita, K., Kikkawa, Y., Kurokawa, K., Doi, Y., 2005. Enzymatic degradation of poly(L-lactide) film by proteinase K quartz crystal microbalance and atomic force microscopy... 

Gan, Z., Liang, Q., Zhang, J., Jing, X., 1997. Enzymatic degradation of poly(E-caprolactone) film in phosphate buffer solution containing lipases. Polymer Degradation and Stability, 56, 209-213. 

Kumagai, Y, Kanesawa, Y. and Doi, Y. (1992) Enzymatic degradation of microbial poly(3-hydroxy butyTate) films, Makromol. Chem., 193, 53- 57. 

L. Liu, S. Li, H. Garreau, M. Vert, Selective enzymatic degradations of poly(l-lactide) and poly(E-eaprolactone) blend films. Biomacromolecules 1(3), 350-359 (2000)... 

Williams reported the enzymatic hydrolysis of poly(L-lactic acid) (PLLA) in the presence of enzymes such as pronase, proteinase K, and bromelain. However, these enzymes are proteases and not PLLA depolymerases [3]. Regarding PLA stereocopolymers, Fukuzaki et al. reported that the hydrolysis of PLA was accelerated in the presence of specific enzymes and the most rapid enzymatic degradation of the stereocopolymers was observed on the poly(DL-lactic acid) (PDLLA) sample containing 50% L-lactic acid (LLA) [4]. Furthermore, Makino et al. showed that the addition of a carboxylic esterase accelerated a decrease in the weight-average molecular weight of PDLLA [5]. Reeve et al. reported the effects of stereochemical composition on enzymatic degradability of PLA films by proteinase K from Tritirachium album [6]. 

Abe H, Doi Y (1999) Stmcturaf effects on enzymatic degradabilities fra- poly [(R)-3-hydroxybutyric acid] and its copolymers. Int J Biol Macromol 25 185-192 Abe H, Khckawa Y, Iwata T, Aoki H, Akehata T, Doi Y (2000) Microscopic visualization on crystalline morphologies of thin films for poly [(R)-3-hydroxybutyric acid] and its copolymer. Pol3Tner 41 867-874... 

Biodegradable Poly(ethylene succinate) (PES). 2. Crystal Morphology of Melt-Crystallized Ultrathin Film and its Change after Enzymatic Degradation, Biomacromolecules,... 

Gan Z, Kuwabara K, Abe H, Iwata T, Doi Y. The role of polymorphic crystal structure and morphology in enzymatic degradation of melt-crystallized poly (butylene adipate) films. Polym Degrad Stab 2005 87 191-199. 

Physical Properties, Structure Analysis, and Enzymatic Degradation of Poly[(i )-3-hydroxybutyrate-co-(i )-3-hydroxyvalerate] Films... 

Fischer JJ, Aoyagi Y, Enoki M, Doi Y and Iwata T, Mechanical properties and enzymatic degradation of poly([R]-3-hydroxybutyrate-co-[R]-3-hydroxy hexanoate) uniaxially cold-drawn films. Polymer Degradation and Stability, 2004, 83 453—460. 

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