Enumeration fungi

Joblin KN (1981) Isolation, enumeration, and maintenance of rumen anaerobic fungi in roll tubes. Appl Environ Microbiol A2 1119-1122. 

Recently, we have developed a novel approach for the rapid enumeration of airborne bacteria and fungi based on SPC. Air samples are collected by impaction on a water soluble polymer that is subsequently dissolved. For labelling of the airborne microorganisms, the viability stain ChemChrome V6 was used (Vanhee et al. 2008, 2009a). 

Hocking, A. D., Pitt, J. I. (1980). Dichloran-glycerol medium for enumeration of xerophilic fungi from low-moisture foods. Applied and Environmental Microbiology, 39,488. 

To control the microbiological situation, sometimes a full count of microbe species at different locations is required, rather than determination of the presence of certain microbial groups like sHme-forming species, fungi, yeasts, anaerobic bacteria, etc. Conventional methods for identification of microbes include enumeration and screening of individual species isolated from count plates on selective media. Ready-made plates are available for the selection of different types of microbes. Commercial identification systems for industry and medical purposes, based on numerous biochemical tests or other characteristics, are available, including a database that covers practically aU groups of bacteria. 

Korburger, J.A. and Farhat, B.Y. (1975). Fungi in Foods VI. A comparison of media to enumerator yeast and molds. Milk Food Techno1. 38, 466-468. 

Put, H.M.C. and Conway, C.C. (1974). The limitations of oxytetracycline for the enumeration of fungi in soil feeds and food in comparison with the selectivity obtained by globenicol (Chloramphenicol). Arch Leben-smittelhyg. 25, 73-83. 

Microorganisms were enumerated by the spread plate technique (Lorch et al., 1995) using tripticasein soy agar (TSA Becton Dickinson, Mexico) for bacteria, and potato dextrose agar (PDA Becton Dickinson, Mexico) with chloramphenicol (Sigma), 100 mg L, for fungi. 

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