Endosperm proteins electrophoresis

ANDERSON ANDERSON High-Resolution, Two-Dimensional Electrophoresis 135 Analysis of Wheat Endosperm Proteins... 

Brown, J.W.S., Law, C.N., Worland, A.J., and Flavell, R.B. Genetic variation in wheat endosperm proteins. An analysis by two-dimensional electrophoresis using intervarietal chromosomal substitution lines. Theor. Appl. Genet. 59 361-371,1981. 

There is a report that in pea embryo, some of the SSSs may also be bound to the starch granule (Denyer et al., 1993) and that in maize endosperm, some of SSSI adheres to the starch granule (Mu et al., 1994). The conclusions in the pea embryo study (Denyer et al., 1993) are based on positive immunoblots obtained after electrophoresis of the SSS with antibody prepared against the GBSS, and also on the similarity of the amino-acid sequence of three peptides obtained from protease SV8 digests of the SSS. This clearly shows there is a close relationship between SSSII and GBSSII, but does not indicate that they are identical proteins. It is also... 

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