Endonuclease VII

ENDONUCLEASE V (PYRIMIDINE DIMER) ENDONUCLEASE VII ENDO-OLIGOPEPTIDASE A Endopeptidase,... 

Double-crossover molecules have been used extensively to characterize the properties of Holliday junctions. The strong torsional coupling between their crossover points has been exploited to construct symmetric immobile junctions (S. Zhang et al. 1993), junctions in which one of the crossovers is flanked by homology, but is nevertheless unable to branch migrate. Symmetric immobile junctions have been used to characterize crossover isomerization thermodynamics (S. Zhang and Seeman 1994) and, more recently, the sequence dependence of the branch point stability (W. Sun et al., 1998). Double crossover molecules have also been employed to establish the cleavage patterns of endonuclease VII, an enzyme that resolves branched junctions (Fu et al. 1994 a). 

Fu, T.-J., Kemper, B., Seeman, N.C. (1994a) Endonuclease VII cleavage of DNA double crossover molecules. Biochemistry 33, 3896-3905. 

In principle the global structure of an RNA junction could be determined when it is complexed with bound protein. This has not been accomplished to date in RNA, but it has proven itself for DNA junctions. For example, the structure of DNA junctions bound by the junctionresolving enzymes T4 endonuclease VII (Pohler et al., 1996) and T7 endonuclease I (Declais et al., 2003) have both been determined by comparative gel electrophoresis. It was found that both proteins substantially alter the global shape of the DNA junction in different ways. These structures were both recendy confirmed by X-ray crystallography (Biertiimpfel et al., 2007 Hadden et al., 2007), showing that comparative gel electrophoresis functions reliably for protein complexes. There is no reason to expect that the method would not work equally well for RNA junction complexes. The binding of the ribosomal protein SI 5 to a three-way RNA junction has been studied by an electrophoretic approach that is related to comparative gel electrophoresis (Batey and Williamson, 1998). 

Youil, R., B. W. Kemper, and R. G. Cotton, Screening for mutations by enzyme mismatch cleavage with T4 endonuclease VII. Proc Natl Acad Sci USA, 1995. 92(1) ... 

Devillers-Thiery A. (1974). Utilisation des endonucleases dans I etude des sequences des ADN. Thesis, Universite Paris VII. 

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