Transmission electron microscopy embryos

Transmission electron microscopy analyses of ultrathin sections have indicated that there are several vacuoles in myrosin cells of embryos. These vacuoles contain myrosinase, as revealed by immunogold labelling studies, and have been referred to as myrosin grains.40, 1 Recently, confocal laser scanning immuno-microscopy analysis with the anti-myrosinase monoclonal antibody 3D7 has been... 

Chapman, A., Blervacq, A.-S., Tissier, J.-P., Delbreil, B., Vasseur, J. Hilbert, J.-L. (2000a). Cell wall differentiation during early somatic embryogenesis in plants. I. Scanning and transmission electron microscopy study on embryos originating from direct, indirect, and adventitious pathways. Canadian Journal of Botany, Vol. 78, No. 6, (June 2000), pp. 816-823, ISSN 0008-4026... 

In addition to labeling NMJs, we have included procedures for transmission electron microscopy (TEM) preparation of NMJs in embryos and first-, second-, and third-instar larvae (see Protocol 11.5). These protocols are not described in detail elsewhere, and the ultrastructure of the NMJ can provide very valuable information about, for example, the number and morphology of active zones, size and number of synaptic vesicles, size of boutons, and alignment of pre- and postsynaptic densities. This information cannot be obtained in any other fashion than by TEM. 

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