Electrophoretic plating

Electrophoretic Plating-the production of a layer of deposit as a result of discharge of colloidal particles in solution onto an electrode. 

Electrophoretic Plating (Electrophoretic Deposition) production of a layer or deposit onto an electrode by discharge of colloidal particles from a solution. 

Animals were inoculated with Enterobacter cloacae and hemolymph was collected as described (11,12). Hemolymph proteins were separated by isoelectric focusing (IEF) on LKB PAG plates at pH 3.5-9.5 according to the manufacturers instructions. To detect antibacterial activity after IEF, the electrophoretic plates were overlaid with phosphate buffered LB agar seeded with E coli strain D31. The antibacterial factors diffused from the IEF plate into the bacteria-containing overlay thus blocking bacterial growth and revealing bands of activity (13). 

First, solutes with larger electrophoretic mobilities (in the same direction as the electroosmotic flow) have greater efficiencies thus, smaller, more highly charged solutes are not only the first solutes to elute, but do so with greater efficiency. Second, efficiency in capillary electrophoresis is independent of the capillary s length. Typical theoretical plate counts are approximately 100,000-200,000 for capillary electrophoresis. 

Stalcup aiid co-workers [14] adapted this method to a continuous elution mini-prep electrophoresis apparatus shown in Fig. 11-3. In this apparatus, the end of the electrophoretic gel is continuously washed with elution buffer. The eluent can then be monitored using an HPLC detector (Fig. 11-4) and sent to a fraction collector where the purified enantiomers, as well as the chiral additive, may be recovered. In this system, the gel configuration was approximately 100 mm x 7 mm, and was aircooled. The number of theoretical plates obtained for 0.5 mg of piperoxan with this gel was approximately 200. A larger, water-cooled gel was able to handle 15 mg of... 

Once an electrophoretic separation has been accomplished, the paper or gel is sprayed or dipped in a visualizing solution similar to that used in the visualization of components on a thin-layer plate. Detection methods similar to those used in HPLC are used in CE. The type of analytes (e.g., inorganic ions, organic ions, and ionic biomolecules) will determine which detection method is best. 

System suitability tests serve to define the level of electrophoretic performance necessary to ensure valid CE assay results. System suitability of the method was evaluated by analyzing the symmetry of the IB-367 peak, theoretical plates of the capillary, and resolution between IB-367 and IB-300, the closest peak to IB-367. The sample concentration of the method was selected at approximately 0.5 mg/ml to assure symmetry below 3.5 and to assume sufficient sensitivity for detecting low... 

From the relationship existing between migration distance and electrophoretic mobility and between peak width and number of theoretical plates, the resolution can be expressed as... 

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