Electrochemical immunoassay advantages

The two modes of performing the electrochemical immunoassay for milk progesterone using SPCE-based immunosensors have advantages and disadvantages. 

The major advantage of electrochemical immunoassays is the fact that it s based on the use of a non-optical detection system, which makes it possible to detect signals in the presence of whole blood samples. There are three different ways in which electrochemistry is used in a detection system. 

Electrochemical immunoassays may offer some advantages over other multiplex sensing technologies, which often require expensive reagents and complex instrumentation. As described in the following sections, the application of nanomaterials to electrochemical immunoassays and other types of electrochemical biosensing devices has resulted in extremely sensitive analytical techniques for multiplexed analyses. 

The amplification of the amperometric response of redox species has been studied for p-aminophenol The sensitive detection of this compound is of particular interest since it is the basis for the measurement of enzymes such as alkaline phosphatase and (J-galactosidase, which are frequently used in enzyme immunoassays The electrode active species (p-aminophenol) is liberated in the enzyme catalysed hydrolysis of its aminophenylated substrate which itself exhibit an irreversible electrochemical behavior The utility of p-aminophenyl phosphate in enzyme immunoassays in combination with IDA electrodes has been demonstrated in [6] The unfavorable pH-optimum of alkaline phosphatase is the drawback of this system Because of its neutral pH-optimum p-galactosidase appeared to be more advantageous An illustration of the proposed electrochemical enzyme immunoassay is given in Fig 2... 

---