Electrical-cell-substrate impedence

Wegener J, Keese CR, Giaever I. Electric cell-substrate impedance sensing (ECIS) as a noninvasive means to monitor the kinetics of cell spreading to artificial surfaces. Exp Cell Res 2000 259 158-66. 

EClS Electric cell-substrate impedance sensing... 

Electrical Cell-substrate Impedance Spectroscopy (ECIS) measurements based on Oscillation-Based Test techniques... 

Figure 10.26 shows an example of cell attachment and motion as measured with the Electric Cell-substrate Impedance Sensing instrument, which is a commercially available version of this system (Applied BioPhysics Inc., Troy, New York, USA). 

Electrical Cell-Substrate Impedance Spectroscopy (EClS)... 

J. Hong, K. Kandasamy, M. Marimuthu, C. Choi, and S. Kim, Electrical cell-substrate impedance sensing as a non-invasive tool for cancer cell study, Analyst, vol. 136, no. 2, pp. 237-245, 2011. 

Xiao C, Luong JHT (2003) On-line monitoring of cell growth and cytotoxicity using electric cell-substrate impedance sensing. Biotechnol Prog 19(3) 1000-1005... 

In another type, mammalian cells or plasma membranes are used as electrical capacitors. Electrical impedance (El) uses the inherent electrical properties of cells to measure the parameters related to the tissue environment (Kyle et al., 1999). The mechanical contact between cell-cell and cell-substrates is measured via conductivity or El (Deng et al., 2003 ... 

The two-half cells contain the aqueous substrate. The mixed monolayers are spread onto the surface of one of the two-hetlf cells. Two "identical" 24lAm 0.7 mCi ionizing electrodes, purchased from the Radiochemical Center Amersham (U.K.), are placed above the hedf cells close to the substrate surface. The difference in electric potenticd of the two electrodes is measured with a high impedence electrometer. By definition this difference is equal to the surface potentietl AV of the monolayer located on the surface on one of the two-hedf cells. The surface potential AV is measured as a function of the spread monolayer molecular area a for each one of the mixed film compositions as above (see surface pressure studies). 

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