Eicosanoids reversed-phase

Analysis of individual lipid species of a class in the Lipid MAPS protocols was largely developed based on reversed-phase LC-MS. For example, in the protocol for eicosanoid analysis, a C18 column (250 X 2.1 mm) was employed and a specific gradient from mobile phase A (water-acetonitrile-formic acid, 63 37 0.02, v/v/v) to B (acetonitrile-isopropanol, 50 50, v/v) was applied [71]. Individual eicosanoid species were detected by using the MRM approach and quantified in comparison to the relevant internal standard. 

Yue, H., Strauss, K.L, Borenstein, M.R., Barbe, M.F., Rossi, L.J. and Jansen, S.A. (2004) Determination of bioactive eicosanoids in brain tissue by a sensitive reversed-phase liquid chromatographic method with fluorescence detection. J. Chromatogr. B 803, 267-277. Puppolo, M., Varma, D. and Jansen, S.A. (2014) A review of analytical methods for eicosanoids in brain tissue. J. Chromatogr. B 964,50-64. 

Another major asset in the routine purification and analysis of the eicosanoids is high-performance liquid chromatography (HPLC). In particular, reversed-phase HPLC has proven to be extremely effective, especially when combined with radioactive, spectroscopic or immunochemical detection methods.For example, the combination of reversed-phase HPLC with ultraviolet absorbance spectroscopy was instrumental to the discovery of leukotrienes and lipoxins, and it is still widely used to detect these compounds as well as other ultraviolet-absorbing eicosanoids. ... 

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