Efficiency, retention, selectivity and resolution in chromatography

The moving phase in liquid chromatography is generally referred to as the mobile phase. In liquid chromatography, the mobile phase flows through or across the stationary phase as a result of a pressure difference (P) across the column. [Pg.16]

Separation of solutes injected into the system arises from differential retention of the solutes by the stationary phase. The net retention of a particular solute depends upon all the solute-solute, solute-mobile phase, solute-stationary phase and stationary phase-mobile phase interactions that contribute to retention. The t3q3es of solute-stationary phase interactions involved in chromatographic retention include hydrogen bonding, van der Waal s forces, electrostatic forces or hydrophobic forces. [Pg.16]