Effects on Streptomyces griseus

Kelner, A. (1949). Effect of visible light on the recovery of Streptomyces griseus conidia from ultraviolet irradiation injury. Proc. Natl. Acad. Sci. USA 35, 73-79. 

The inhibitory effect of inorganic phosphate on biosynthetic pathways of secondary metabolites including non--phosphorylated intermediates is explained less readily. The action usually involves the derepression of synthesis of enzymes which carry out the formation of secondary products after the inorganic phosphate has been exhausted (4). The study by Martin et al. (23) of the effect of orthophosphate on candicidin biosynthesis in Streptomyces griseus suggested that inorganic phosphate either inhibits the activity of candicidin synthases or represses their synthesis (24). Inorganic phosphate also represses the synthesis of anhydrotetracycline hydratase in Streptomyces aureofaciens while benzyl thiocyanate abolishes its inhibitory effect (4). 

Shaw, R. K., R. D. Henderson, and W. J. Seagers Effect of cations on manno-sidostreptomycinase and streptomycin production by Streptomyces griseus. Appl. Microbiol. 8, 12 (I960). 

In a previous study, we incubated chromatophores of Rps.caps. with a specific pronase (type III, Sigma) from Streptomyces griseus (5). The effect of pronase digestion on the spectral characteristics of both the field-sensitive and the field-insensitive carotenoid pools was monitored. It was shown that the field-responding carotenoids are more sensitive to pronase treatment than the non-responding pool. In the present study, these experiments were extended to other digestive treatments of chromatophores of Rhodopseudomonas capsulata as well as sphaeroides. 

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