Effective and Inhibitory Concentrations

The maximum in this curve is a plateau at a value equal to radioligand binding in the absence of the competing unlabeled drug (total binding), whereas the minimum 

Nonlinear regression is nsed to fit the competitive binding curve to determine the log(IC5o). Three factors determine the valne of ICjq  

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Effective and inhibitory concentrations correspond to apparent dissociation constants of the host-guest complexes. These values can be quite different from the true Kd values. Particularly cautious interpretation is recommended for stoichiometric binding, molecular recognition may actually be much better than it appears in these cases [43-45]. Quantitative correlation of the values of JC50 and KD [44] is not common in the field of synthetic ion channels and pores. The Hill coefficient n... 

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