Effect of Temperature on Insect Cell Growth Kinetics

2 Effect of Temperature on Insect Cell Growth Kinetics 

Andersen et al. (1996) and Andersen (1995) have studied the effect of temperature on the recombinant protein production using a baulovinis/insect cell expression system. In Tables 17.15, 17.16, 17.17, 17.18 and 17.19 we reproduce the growth data obtained in spinner flasks (batch cultures) using Bombyx mori (Bm5) cells adapted to serum-free media (Ex-Cell 400). The working volume was 125 ml and samples were taken twice daily. The cultures were carried out at six different incubation temperatures (22, 26,28, 30 and 32 TT). 

In the above tables the following notation has been used t Elapsed Time (/ ) 

At each temperature the simple Monod kinetic model can be used that can be combined with material balances to arrive at the following unstructured model 

The limiting substrate (glucose) concentration is denoted by S. There are four parameters pmax is the maximum specific growth rate, Ks is the saturation constant for S, kd is the specific death rate and Y is the average yield coefficient (assumed constant). 

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